Anti-PCNA Recombinant Antibody (SAA2241) (HY337013)

Name: Anti-PCNA Recombinant Antibody (SAA2241)
Brand: abinScience
SKU: HY337013
Price: 370 USD
Availability: InStock

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Precio(USD):

Especificación:

100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY337013

Reactividad de especies

Human

Aplicaciones

ELISA, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

SAA2241

Objetivo

PCNA

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>90% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P12004

RRID

Anti-PCNA Antibody (SAA2241) (abinScience Cat# HY337013, RRID:AB_3729877)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

Cyclin, Proliferating cell nuclear antigen, PCNA

Fondo

DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737

2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902

3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat spleen lysate
Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PCNA (Catalog No: HY337012)
Lane 2: negative control
Bioactivity

Detects recombinant human PCNA (Catalog No: HY337012) in indirect ELISA