Anti-PCNA Recombinant Antibody (SAA2241) (HY337013)

Name: Anti-PCNA Recombinant Antibody (SAA2241)
Brand: abinScience
SKU: HY337013
Price: 370 USD
Availability: InStock

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Prix(USD) :

Spécification :

100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY337013

Reactivité des espèces

Human

Applications

ELISA, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

SAA2241

Cible

PCNA

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>90% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P12004

RRID

Anti-PCNA Antibody (SAA2241) (abinScience Cat# HY337013, RRID:AB_3729877)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Noms alternatifs

Cyclin, Proliferating cell nuclear antigen, PCNA

Contexte

DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737

2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902

3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat spleen lysate
Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PCNA (Catalog No: HY337012)
Lane 2: negative control
Bioactivity

Detects recombinant human PCNA (Catalog No: HY337012) in indirect ELISA