Anti-PCNA Recombinant Antibody (SAA2241) (HY337013)

Name: Anti-PCNA Recombinant Antibody (SAA2241)
Brand: abinScience
SKU: HY337013
Price: 370 USD
Availability: InStock

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Цена(USD):

Спецификация:

100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HY337013

Реактивность видов

Human

Применения

ELISA, WB

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

SAA2241

Цель

PCNA

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>90% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P12004

RRID

Anti-PCNA Antibody (SAA2241) (abinScience Cat# HY337013, RRID:AB_3729877)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Альтернативные названия

Cyclin, Proliferating cell nuclear antigen, PCNA

Фон

DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737

2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902

3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat spleen lysate
Western blot

Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PCNA (Catalog No: HY337012)
Lane 2: negative control
Bioactivity

Detects recombinant human PCNA (Catalog No: HY337012) in indirect ELISA