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| カタログ番号 | HA574014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-PDHA2 Polyclonal Antibody (HA574014) is a rabbit polyclonal antibody detecting PDHA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Rat, and Pig.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human PDHA2 (Ser28-Ser388). | ||||||||
| ターゲット | Pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial, EC:1.2.4.1, PDHE1-A type II, PDHA2 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 観測分子量 | ~43 kDa | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P29803, P35486, , A7MB35, P26284, P29804, P08559 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (PDHA2) is a ~42 kDa protein. Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex in testis. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2. 1. Hiromasa, Y. et al. (2004) The Journal of biological chemistry 279, 6921-33. PMID: 14638692 2. Korotchkina, LG. et al. (2006) The Journal of biological chemistry 281, 9688-96. PMID: 16436377 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-PDHA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat kidney lysate
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