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| カタログ番号 | HS822014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-PDK1 Polyclonal Antibody (HS822014) is a rabbit polyclonal antibody detecting PDK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 種反応性 | Human, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human PDK1 (Tyr233-Met430). | ||||||||
| ターゲット | PDHK1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q15118 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) is a ~49 kDa protein. Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. 1. Gudi, R. et al. (1995) The Journal of biological chemistry 270, 28989-94. PMID: 7499431 2. McFate, T. et al. (2008) The Journal of biological chemistry 283, 22700-8. PMID: 18541534 3. Hitosugi, T. et al. (2011) Molecular cell 44, 864-77. PMID: 22195962 4. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675 5. Kato, M. et al. (2007) Structure (London, England : 1993) 15, 992-1004. PMID: 17683942 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-PDK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Lncap cell lystae
Lane 5: Hela cell lysate
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