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Anti-PDLIM4 Polyclonal Antibody (HA712014)

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Anti-PDLIM4 Polyclonal Antibody
Anti-PDLIM4 Polyclonal Antibody
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHA712014
Descripción
Anti-PDLIM4 Polyclonal Antibody (HA712014) is a rabbit polyclonal antibody detecting PDZ and LIM domain protein 4; LIM protein RIL; Reversion-induced LIM protein; PDLIM4; RIL in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human PDLIM4 (Pro2-Glu84).
Objetivo PDZ and LIM domain protein 4; LIM protein RIL; Reversion-induced LIM protein; PDLIM4; RIL
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P50479, P70271, P36202, Q3T005
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

PDZ and LIM domain protein 4 (PDLIM4) is a ~35 kDa protein. Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton. In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane.

1. Zhang, Y. et al. (2009) The Journal of cell biology 184, 785-92. PMID: 19307596
2. Guryanova, OA. et al. (2011) The Journal of biological chemistry 286, 26849-59. PMID: 21636573
Nota For research use only
Imágenes
  • Anti-PDLIM4 Polyclonal Antibody

    Western blot

    Lane 1: A431 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Mouse brain lysate
    Lane 4: Rat brain lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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