Anti-PEDV Spike glycoprotein S1 Polyclonal Antibody (VK564054)

Name: Anti-PEDV Spike glycoprotein S1 Polyclonal Antibody
Brand: abinScience
SKU: VK564054
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK564054

Description

Anti-PEDV S1/Spike glycoprotein 1 Polyclonal Antibody (VK564054) is a rabbit polyclonal antibody detecting Spike glycoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Porcine epidemic diarrhea virus (PEDV)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant PEDV S1/Spike glycoprotein 1 (Gln21-Glu496).

Applications testées

Cible

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S, Porcine epidemic diarrhea virus, PEDV

Purification

Purified by antigen affinity column.

Numéro d'accès

S0AP48

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Spike glycoprotein (S1/Spike glycoprotein 1) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-S1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant PEDV S1/Spike glycoprotein 1 (Catalog No: VK564012)

Reférences

Fiche technique