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Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody (VK564074)

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Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody
Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody
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Aperçu
Numéro de catalogueVK564074
Description
Anti-PEDV Spike Glycoprotein S2 Polyclonal Antibody (VK564074) is a rabbit polyclonal antibody detecting Spike glycoprotein. Suitable for Porcine epidemic diarrhea virus.
Reactivité des espècesPorcine epidemic diarrhea virus (PEDV)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant PEDV Spike Glycoprotein S2 (Gly777-Val1120).
Applications testées WB
Cible E2, Peplomer protein, S, S glycoprotein, Spike glycoprotein
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q91AV1
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Spike glycoprotein (Spike Glycoprotein S2) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note For research use only
Images
  • Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody

    Western blot

    Lane 1: recombinant PEDV Spike Glycoprotein S2, N-His (Catalog No: VK564052)

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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Anti-PEDV Spike glycoprotein S2 Polyclonal Antibody

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