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Anti-PEPD Polyclonal Antibody (HD441014)

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Anti-PEPD Polyclonal Antibody
Anti-PEPD Polyclonal Antibody
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Aperçu
Numéro de catalogueHD441014
Description
Anti-PEPD Polyclonal Antibody (HD441014) is a rabbit polyclonal antibody detecting PEPD in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PEPD (Thr188-Lys493).
Cible EC:3.4.13.9, Imidodipeptidase, PEPD, PRD, Peptidase D, Prolidase, Proline dipeptidase, X-Pro dipeptidase, Xaa-Pro dipeptidase
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P12955
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsP12955
Contexte

Xaa-Pro dipeptidase (PEPD) is a ~54 kDa protein. Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position. The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

1. Lupi, A. et al. (2006) The FEBS journal 273, 5466-78. PMID: 17081196
2. Rao, SD. et al. (2022) Nature chemical biology 18, 565-574. PMID: 35165443
3. Endo, F. et al. (1989) The Journal of biological chemistry 264, 4476-81. PMID: 2925654
Note For research use only
Images
  • Anti-PEPD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PEPD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: SH-SY5Y cell lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PEPD Polyclonal Antibody

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