Anti-Human CXCL4/PF4 Polyclonal Antibody (HY443014)

Name: Anti-Human CXCL4/PF4 Polyclonal Antibody
Brand: abinScience
SKU: HY443014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY443014

Descripción

Anti-PF4/CXCL4 Polyclonal Antibody (HY443014) is a rabbit polyclonal antibody detecting CXCL4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PF4/CXCL4 (Glu32-Ser101).

Objetivo

C-X-C motif chemokine 4, PF-4, Oncostatin-A, Endothelial cell growth inhibitor, CXCL4, Iroplact, Platelet factor 4, PF4, SCYB4

Purificación

Purified by antigen affinity column.

Número de acceso

P02776

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Platelet factor 4 (CXCL4/PF4) is a ~10 kDa protein. Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence.

1. Fox, JM. et al. (2018) Scientific reports 8, 9466. PMID: 29930254

2. Lecomte-Raclet, L. et al. (1998) Blood 91, 2772-80. PMID: 9531587

3. Mueller, A. et al. (2008) Journal of leukocyte biology 83, 875-82. PMID: 18174362

4. Van Raemdonck, K. et al. (2014) Angiogenesis 17, 631-40. PMID: 24469069

5. Yang, C. et al. (2022) Nature communications 13, 3426. PMID: 35701499

6. Affandi, AJ. et al. (2022) Cell reports 38, 110189. PMID: 34986347

7. Dudek, AZ. et al. (1997) The Journal of biological chemistry 272, 31785-92. PMID: 9395524

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-PF4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate
Lane 2: Mouse heart lysate

Referencias

Datasheet

Anti-Human CXCL4/PF4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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