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Anti-PHF2 Polyclonal Antibody (HB551014)

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Aperçu
Numéro de catalogueHB551014
Description
Anti-PHF2 Polyclonal Antibody (HB551014) is a rabbit polyclonal antibody detecting Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human PHF2 (Gln83-Arg450).
Cible Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès O75151, Q9WTU0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Lysine-specific demethylase PHF2 is a ~120 kDa protein. Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

1. Wen, H. et al. (2010) The Journal of biological chemistry 285, 9322-9326. PMID: 20129925
2. Horton, JR. et al. (2011) Journal of molecular biology 406, 1-8. PMID: 21167174
3. Baba, A. et al. (2011) Nature cell biology 13, 668-75. PMID: 21532585
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Working Solution
C₂ (Working Conc.)
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Anti-PHF2 Polyclonal Antibody

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