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| カタログ番号 | HB683013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | IHC, IP, WB | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a, kappa | ||||
| クローンID | 1stS2 | ||||
| ターゲット | DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, POLR2A, POLR2, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P24928 | ||||
| RRID | Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Antibody (1stS2) (abinScience Cat# HB683013, RRID:AB_3725749) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | DNA-directed RNA polymerase II subunit RPB1 (p-POLR2A) is a ~217 kDa protein. Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA. 1. Kassube, SA. et al. (2013) Nature structural & molecular biology 20, 892-9. PMID: 23748380 2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682 3. Jishage, M. et al. (2018) Nature structural & molecular biology 25, 859-867. PMID: 30190596 4. Kershnar, E. et al. (1998) The Journal of biological chemistry 273, 34444-53. PMID: 9852112 5. Vo Ngoc, L. et al. (2017) Genes & development 31, 6-11. PMID: 28108474 6. Wang, D. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 843-7. PMID: 8381534 8. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Antibody (1stS2)
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