Anti-Phospho-Tau (pS396/pS404) Recombinant Antibody (SAA0295) (HY086083)

Name: Anti-Phospho-Tau (pS396/pS404) Recombinant Antibody (SAA0295)
Brand: abinScience
SKU: HY086083
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY086083

Reactividad de especies

Human

Aplicaciones

IHC, IP, WB, FCM

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

SAA0295

Aplicaciones probadas

FCM

Objetivo

MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P10636

RRID

Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295) (abinScience Cat# HY086083, RRID:AB_3725735)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Microtubule-associated protein tau (P-Tau) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311

2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270

Nota

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295).
Flow CytoMetry

Flow-cytometry using APC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.