Anti-Phospho-Tau (pS396/pS404) Recombinant Antibody (SAA0295) (HY086083)

Name: Anti-Phospho-Tau (pS396/pS404) Recombinant Antibody (SAA0295)
Brand: abinScience
SKU: HY086083
Price: 126 USD
Availability: InStock

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20ug
50ug
100ug
1mg

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HY086083

Реактивность видов

Human

Применения

IHC, IP, WB, FCM

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a, kappa

ID клона

SAA0295

Проверенные приложения

FCM

Цель

MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P10636

RRID

Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295) (abinScience Cat# HY086083, RRID:AB_3725735)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4.

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Информация об использовании продукта

Применение	Разбавление
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Microtubule-associated protein tau (P-Tau) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311

2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270

Примечание

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Изображения

SDS-PAGE

SDS-PAGE for Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295).
Flow CytoMetry

Flow-cytometry using APC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.