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Anti-Phosphoprotein Polyclonal Antibody (VK078014)

Anti-Phosphoprotein Polyclonal Antibody
Anti-Phosphoprotein Polyclonal Antibody
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概要
カタログ番号VK078014
説明
Anti-Phosphoprotein Polyclonal Antibody (VK078014) is a rabbit polyclonal antibody detecting P phosphoprotein in ELISA, IHC, WB. Suitable for Nipah virus and Hendra virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Nipah virus (NiV), Hendra virus (HeV)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant nipah virus Phosphoprotein (Asp471-Gly580).
ターゲット P phosphoprotein, partial; P/V/W/C
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 O55778, Q9IK91
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Phosphoprotein is a ~78 kDa protein. Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis. Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication. The nucleoprotein protein N prevents excessive phosphorylation of P, which leads to down-regulation of viral transcription/ replication. Participates, together with N, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place.

注意事項 For research use only
イメージ
  • Anti-Phosphoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-P polyclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG16620 100ng

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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