Anti-PIGR Polyclonal Antibody (HF948014)

Name: Anti-PIGR Polyclonal Antibody
Brand: abinScience
SKU: HF948014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Vista general

Número de catálogo

HF948014

Descripción

Anti-PIGR Polyclonal Antibody (HF948014) is a rabbit polyclonal antibody detecting PIgR in ELISA, IHC, WB. Suitable for Capra hircus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PIGR (Arg289-Arg638).

Objetivo

Poly-Ig receptor, Polymeric immunoglobulin receptor, PIgR, PIGR, Hepatocellular carcinoma-associated protein TB6

Purificación

Purified by antigen affinity column.

Número de acceso

P01833

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Polymeric immunoglobulin receptor (PIGR) is a ~83 kDa protein. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Nota

For research use only.

Imágenes

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting PIGR (HF948014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of PIGR was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-PIGR polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HL60 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MOLT-4 cell lysate