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Anti-PIK3C2A Polyclonal Antibody (HT279014)

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Vista general
Número de catálogoHT279014
Descripción
Anti-PIK3C2A Polyclonal Antibody (HT279014) is a rabbit polyclonal antibody detecting PIK3C2A in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PIK3C2A (Phe172-Asn403).
Objetivo Phosphoinositide 3-kinase-C2-alpha, PIK3C2A, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PtdIns-3-kinase C2 subunit alpha, PI3K-C2-alpha
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O00443
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PIK3C2A) is a ~190 kDa protein. Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion.

1. Tiosano, D. et al. (2019) PLoS genetics 15, e1008088. PMID: 31034465
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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