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Anti-PITRM1 Polyclonal Antibody (HV687014)

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Anti-PITRM1 Polyclonal Antibody
Anti-PITRM1 Polyclonal Antibody
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Vista general
Número de catálogoHV687014
Descripción
Anti-PITRM1 Polyclonal Antibody (HV687014) is a rabbit polyclonal antibody detecting PITRM1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PITRM1 (Leu544-Ser806).
Objetivo Pitrilysin metalloproteinase 1, hMP1, MP1, Metalloprotease 1, PREP, KIAA1104, Presequence protease, mitochondrial, PITRM1, hPreP
Purificación Purified by antigen affinity column.
Número de acceso Q5JRX3
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Presequence protease, mitochondrial (PITRM1) is a ~117 kDa protein. Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage.

1. Mzhavia, N. et al. (1999) DNA and cell biology 18, 369-80. PMID: 10360838
2. Falkevall, A. et al. (2006) The Journal of biological chemistry 281, 29096-104. PMID: 16849325
3. Chow, KM. et al. (2009) Biochemistry 48, 2868-77. PMID: 19196155
4. King, JV. et al. (2014) Structure (London, England : 1993) 22, 996-1007. PMID: 24931469
Nota For research use only.
Imágenes
  • Anti-PITRM1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PITRM1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MCF7 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Mouse kidney lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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