Anti-PITRM1 Polyclonal Antibody (HV687014)

Name: Anti-PITRM1 Polyclonal Antibody
Brand: abinScience
SKU: HV687014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HV687014

説明

Anti-PITRM1 Polyclonal Antibody (HV687014) is a rabbit polyclonal antibody detecting PITRM1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human PITRM1 (Leu544-Ser806).

ターゲット

Pitrilysin metalloproteinase 1, hMP1, MP1, Metalloprotease 1, PREP, KIAA1104, Presequence protease, mitochondrial, PITRM1, hPreP

精製

Purified by antigen affinity column.

アクセッション番号

Q5JRX3

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Presequence protease, mitochondrial (PITRM1) is a ~117 kDa protein. Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage.

1. Mzhavia, N. et al. (1999) DNA and cell biology 18, 369-80. PMID: 10360838

2. Falkevall, A. et al. (2006) The Journal of biological chemistry 281, 29096-104. PMID: 16849325

3. Chow, KM. et al. (2009) Biochemistry 48, 2868-77. PMID: 19196155

4. King, JV. et al. (2014) Structure (London, England : 1993) 22, 996-1007. PMID: 24931469

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-PITRM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: 293T cell lysate
Lane 3: Mouse kidney lysate

参考文献

データシート

Anti-PITRM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-PITRM1 Polyclonal Antibody (HV687014)

概要

イメージ

参考文献

データシート

SDS