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Anti-PITRM1 Polyclonal Antibody
Anti-PITRM1 Polyclonal Antibody
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Обзор
Каталожный номерHV687014
Описание
Anti-PITRM1 Polyclonal Antibody (HV687014) is a rabbit polyclonal antibody detecting PITRM1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human PITRM1 (Leu544-Ser806).
Цель Pitrilysin metalloproteinase 1, hMP1, MP1, Metalloprotease 1, PREP, KIAA1104, Presequence protease, mitochondrial, PITRM1, hPreP
Очистка Purified by antigen affinity column.
Номер доступа Q5JRX3
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Presequence protease, mitochondrial (PITRM1) is a ~117 kDa protein. Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage.

1. Mzhavia, N. et al. (1999) DNA and cell biology 18, 369-80. PMID: 10360838
2. Falkevall, A. et al. (2006) The Journal of biological chemistry 281, 29096-104. PMID: 16849325
3. Chow, KM. et al. (2009) Biochemistry 48, 2868-77. PMID: 19196155
4. King, JV. et al. (2014) Structure (London, England : 1993) 22, 996-1007. PMID: 24931469
Примечание For research use only.
Изображения
  • Anti-PITRM1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PITRM1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MCF7 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Mouse kidney lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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