Anti-PKP1 Polyclonal Antibody (HC672014)

Name: Anti-PKP1 Polyclonal Antibody
Brand: abinScience
SKU: HC672014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Vista general

Número de catálogo

HC672014

Descripción

Anti-PKP1 Polyclonal Antibody (HC672014) is a rabbit polyclonal antibody detecting PKP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PKP1 (Asn500-Asn742).

Objetivo

B6P, Plakophilin-1, Band 6 protein, PKP1

Purificación

Purified by antigen affinity column.

Número de acceso

Q13835

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Plakophilin-1 (PKP1) is a ~82 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments. Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG. Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions. Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes.

1. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369

2. Hofmann, I. et al. (2000) Journal of cell science 113 ( Pt 13), 2471-83. PMID: 10852826

3. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537

4. McGrath, JA. et al. (1997) Nature genetics 17, 240-4. PMID: 9326952

5. Sobolik-Delmaire, T. et al. (2010) The Journal of investigative dermatology 130, 2638-46. PMID: 20613778

6. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963

7. Fischer-Kešo, R. et al. (2014) Molecular and cellular biology 34, 4244-56. PMID: 25225333

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-PKP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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