Anti-PLA2G6 Polyclonal Antibody (HT140014)

Name: Anti-PLA2G6 Polyclonal Antibody
Brand: abinScience
SKU: HT140014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HT140014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human PLA2G6 (Asp484-Val701).

Cible

Patatin-like phospholipase domain-containing protein 9, Group VI phospholipase A2, iPLA2-beta, PLA2G6, PLPLA9, Intracellular membrane-associated calcium-independent phospholipase A2 beta, GVI PLA2, CaI-PLA2, Palmitoyl-CoA hydrolase, 2-lysophosphatidylcholine acylhydrolase, 85/88 kDa calcium-independent phospholipase A2, PNPLA9

Purification

Purified by antigen affinity column.

Numéro d'accès

O60733

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

85/88 kDa calcium-independent phospholipase A2 (PLA2G6) is a ~89 kDa protein. Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content.

1. Ma, Z. et al. (1999) The Journal of biological chemistry 274, 9607-16. PMID: 10092647

2. Larsson Forsell, PK. et al. (1999) European journal of biochemistry 262, 575-85. PMID: 10336645

3. Engel, LA. et al. (2010) PloS one 5, e12897. PMID: 20886109

4. Larsson, PK. et al. (1998) The Journal of biological chemistry 273, 207-14. PMID: 9417066

5. Hsu, YH. et al. (2013) PloS one 8, e59267. PMID: 23533611

Note

For research use only.

Images

Reférences

Fiche technique

Anti-PLA2G6 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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