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Anti-PNMT Polyclonal Antibody (HB552014)

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Vista general
Número de catálogoHB552014
Descripción
Anti-PNMT Polyclonal Antibody (HB552014) is a rabbit polyclonal antibody detecting Phenylethanolamine N-methyltransferase; PNMTase; 2.1.1.28; Noradrenaline N-methyltransferase; PNMT; PENT in ELISA, IHC, WB. Suitable for Human, Pig, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human PNMT (Ser16-Leu282).
Objetivo Phenylethanolamine N-methyltransferase; PNMTase; 2.1.1.28; Noradrenaline N-methyltransferase; PNMT; PENT
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P11086, Q06AU9, P40935, P10937, P10938
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Phenylethanolamine N-methyltransferase (PNMT) is a ~30 kDa protein. Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine.

1. Kang, DI. et al. (2010) Molecules and cells 29, 595-602. PMID: 20496117
2. Ji, Y. et al. (2005) Journal of neurochemistry 95, 1766-76. PMID: 16277617
3. Gee, CL. et al. (2005) Biochemistry 44, 16875-85. PMID: 16363801
4. Caine, JM. et al. (1996) Protein expression and purification 8, 160-6. PMID: 8812853
Nota For research use only
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PNMT Polyclonal Antibody

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