Anti-POLD1 Polyclonal Antibody (HB094014)

Name: Anti-POLD1 Polyclonal Antibody
Brand: abinScience
SKU: HB094014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HB094014

Description

Anti-POLD1 Polyclonal Antibody (HB094014) is a rabbit polyclonal antibody detecting POLD1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human POLD1 (Met41-Phe254).

Cible

3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, DNA polymerase delta catalytic subunit, POLD

Purification

Purified by antigen affinity column.

Numéro d'accès

P28340

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA polymerase delta catalytic subunit (POLD1) is a ~123 kDa protein. As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4.

1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448

2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196

3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433

4. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480

5. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200

6. Conde, CD. et al. (2019) The Journal of clinical investigation 129, 4194-4206. PMID: 31449058

7. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374

8. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611

9. Maga, G. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 18850-5. PMID: 24191025

Note

For research use only.

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Reférences

Fiche technique

Anti-POLD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-POLD1 Polyclonal Antibody (HB094014)

Aperçu

Images

Reférences

Fiche technique

SDS