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| カタログ番号 | HB094014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-POLD1 Polyclonal Antibody (HB094014) is a rabbit polyclonal antibody detecting POLD1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human POLD1 (Met41-Phe254). | ||||||||
| ターゲット | 3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, DNA polymerase delta catalytic subunit, POLD | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P28340 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA polymerase delta catalytic subunit (POLD1) is a ~123 kDa protein. As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. 1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 4. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480 5. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 6. Conde, CD. et al. (2019) The Journal of clinical investigation 129, 4194-4206. PMID: 31449058 7. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374 8. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611 9. Maga, G. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 18850-5. PMID: 24191025 | ||||||||
| 注意事項 | For research use only. |
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