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Anti-POLD2 Polyclonal Antibody (HA706014)

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Anti-POLD2 Polyclonal Antibody
Anti-POLD2 Polyclonal Antibody
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  • 20ug
  • 50ug
  • 100ug
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Vista general
Número de catálogoHA706014
Descripción
Anti-POLD2 Polyclonal Antibody (HA706014) is a rabbit polyclonal antibody detecting DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; POLD2 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human POLD2 (Met1-Pro469).
Objetivo DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; POLD2
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P49005, P49004, O35654, Q6AXY4
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DNA polymerase delta subunit 2 (POLD2) is a ~51 kDa protein. Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4.

1. Zhang, S. et al. (2007) The Journal of biological chemistry 282, 15330-40. PMID: 17317665
2. Chea, J. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2885-95. PMID: 22801543
3. Lee, YS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 2954-9. PMID: 24449906
4. Xie, B. et al. (2002) Biochemistry 41, 13133-42. PMID: 12403614
5. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448
6. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196
7. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433
8. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200
9. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374
10. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611
Nota For research use only
Imágenes
  • Anti-POLD2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-POLD2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: 293T cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: HL-60 cell lysate
    Lane 6: HepG2 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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