Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

Name: Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody
Brand: abinScience
SKU: JN089014
Price: 168 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN089014

Reactivité des espèces

Porphyromonas gingivalis

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Porphyromonas gingivalis kgp/prtK (Asp229-Pro683).

Cible

Lys-gingipain W83, 3.4.22.47, Lysine specific cysteine protease, Lysine-specific cysteine proteinase, Porphypain, PrtK48, Lys-gingipain catalytic subunit, 39 kDa adhesin, PrtK39, 15 kDa adhesin, PrtK15, 44 kDa adhesin, PrtK44, kgp, prtK, prtP

Purification

Purified by antigen affinity column.

Numéro d'accès

Q51817

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Lys-gingipain W83 (kgp/prtK) is a ~187 kDa protein. Cysteine proteinase with a strong preference for substrates with Lys in the P1 position. Hydrolyzes bovine hemoglobin, bovine serum albumin, casein, human placental type I collagen and human IgA and IgG. Disrupts the functions of polymorphonuclear leukocytes. May act as a virulence factor in the development of peridontal disease. Involved in the coaggregation of P.gingivalis with other oral bacteria.

1. Bhogal, PS. et al. (1997) Microbiology (Reading, England) 143 ( Pt 7), 2485-2495. PMID: 9245829

2. Li, N. et al. (2011) Molecular microbiology 81, 1358-73. PMID: 21812842

3. Ganuelas, LA. et al. (2013) European journal of microbiology & immunology 3, 152-62. PMID: 24265933

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Kgp polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 70 ug
Lane 2: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 50 ug
Lane 3: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 30 ug
Lane 4: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 10 ug

Reférences

Fiche technique

Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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