Anti-PPARG Polyclonal Antibody (HW678014)

Name: Anti-PPARG Polyclonal Antibody
Brand: abinScience
SKU: HW678014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HW678014

Descripción

Anti-PPARG Polyclonal Antibody (HW678014) is a rabbit polyclonal antibody detecting PPARG in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PPARG (Asp230-Tyr505).

Objetivo

NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG

Purificación

Purified by antigen affinity column.

Número de acceso

P37231

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses.

1. Yin, Y. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 268-78. PMID: 16150867

2. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347

3. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231

4. Elbrecht, A. et al. (1996) Biochemical and biophysical research communications 224, 431-7. PMID: 8702406

5. Lambe, KG. et al. (1996) European journal of biochemistry 239, 1-7. PMID: 8706692

6. Mukherjee, R. et al. (1997) The Journal of biological chemistry 272, 8071-6. PMID: 9065481

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-PPARG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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