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Anti-PPIE Polyclonal Antibody (HV182014)

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Vista general
Número de catálogoHV182014
Descripción
Anti-PPIE Polyclonal Antibody (HV182014) is a rabbit polyclonal antibody detecting PPIE in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PPIE (Tyr41-Val264).
Objetivo Peptidyl-prolyl cis-trans isomerase E, PPIE, PPIase E, Cyclophilin-33, CYP33, Rotamase E, Cyclophilin E
Purificación Purified by antigen affinity column.
Número de acceso Q9UNP9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Peptidyl-prolyl cis-trans isomerase E (PPIE) is a ~33 kDa protein. Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638
2. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346
3. Wang, Y. et al. (2008) FEBS letters 582, 835-9. PMID: 18258190
4. Hom, RA. et al. (2010) Journal of molecular biology 400, 145-54. PMID: 20460131
5. Park, S. et al. (2010) Biochemistry 49, 6576-86. PMID: 20677832
6. Mi, H. et al. (1996) FEBS letters 398, 201-5. PMID: 8977107
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PPIE Polyclonal Antibody

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