Anti-PPIE Polyclonal Antibody (HV182014)

Name: Anti-PPIE Polyclonal Antibody
Brand: abinScience
SKU: HV182014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HV182014

説明

Anti-PPIE Polyclonal Antibody (HV182014) is a rabbit polyclonal antibody detecting PPIE in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human PPIE (Tyr41-Val264).

ターゲット

Peptidyl-prolyl cis-trans isomerase E, PPIE, PPIase E, Cyclophilin-33, CYP33, Rotamase E, Cyclophilin E

精製

Purified by antigen affinity column.

アクセッション番号

Q9UNP9

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Peptidyl-prolyl cis-trans isomerase E (PPIE) is a ~33 kDa protein. Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

2. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346

3. Wang, Y. et al. (2008) FEBS letters 582, 835-9. PMID: 18258190

4. Hom, RA. et al. (2010) Journal of molecular biology 400, 145-54. PMID: 20460131

5. Park, S. et al. (2010) Biochemistry 49, 6576-86. PMID: 20677832

6. Mi, H. et al. (1996) FEBS letters 398, 201-5. PMID: 8977107

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-PPIE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-PPIE Polyclonal Antibody (HV182014)

概要

イメージ

参考文献

データシート

SDS