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Anti-PPIF Polyclonal Antibody (HB283014)

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Aperçu
Numéro de catalogueHB283014
Description
Anti-PPIF Polyclonal Antibody (HB283014) is a rabbit polyclonal antibody detecting PPIF in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PPIF (Asn45-Lys196).
Cible CypD, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, Cyclophilin D, Cyclophilin F, CYP3, CyP-M, Rotamase F, PPIF, CyP-D, PPIase F, Mitochondrial cyclophilin
Purification Purified by antigen affinity column.
Numéro d'accès P30405
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIF) is a ~22 kDa protein. PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels.

1. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357
2. Shanmughapriya, S. et al. (2015) Molecular cell 60, 47-62. PMID: 26387735
3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440
4. Eliseev, RA. et al. (2009) The Journal of biological chemistry 284, 9692-9. PMID: 19228691
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-PPIF Polyclonal Antibody

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