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Каталожный номерHB283014
Описание
Anti-PPIF Polyclonal Antibody (HB283014) is a rabbit polyclonal antibody detecting PPIF in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human PPIF (Asn45-Lys196).
Цель CypD, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, Cyclophilin D, Cyclophilin F, CYP3, CyP-M, Rotamase F, PPIF, CyP-D, PPIase F, Mitochondrial cyclophilin
Очистка Purified by antigen affinity column.
Номер доступа P30405
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIF) is a ~22 kDa protein. PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels.

1. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357
2. Shanmughapriya, S. et al. (2015) Molecular cell 60, 47-62. PMID: 26387735
3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440
4. Eliseev, RA. et al. (2009) The Journal of biological chemistry 284, 9692-9. PMID: 19228691
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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