Anti-PPP3CA Polyclonal Antibody (HC519014)

Name: Anti-PPP3CA Polyclonal Antibody
Brand: abinScience
SKU: HC519014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC519014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human PPP3CA (Ile7-Ser301).

Cible

CAM-PRP catalytic subunit, CNA, Calmodulin-dependent calcineurin A subunit alpha isoform, CALNA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, PPP3CA

Purification

Purified by antigen affinity column.

Numéro d'accès

Q08209

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Protein phosphatase 3 catalytic subunit alpha (PPP3CA) is a ~58 kDa protein. Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals.

1. Wang, Y. et al. (2005) The Journal of biological chemistry 280, 12683-9. PMID: 15671020

2. Cereghetti, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15803-8. PMID: 18838687

3. Kilka, S. et al. (2009) Biochemistry 48, 1900-10. PMID: 19154138

4. Grigoriu, S. et al. (2013) PLoS biology 11, e1001492. PMID: 23468591

5. Rydzanicz, M. et al. (2019) European journal of human genetics : EJHG 27, 61-69. PMID: 30254215

6. Li, H. et al. (2007) Journal of molecular biology 369, 1296-306. PMID: 17498738

7. Takeuchi, K. et al. (2007) Structure (London, England : 1993) 15, 587-97. PMID: 17502104

8. Li, H. et al. (2012) Nature structural & molecular biology 19, 337-45. PMID: 22343722

9. Sheftic, SR. et al. (2016) Scientific reports 6, 38920. PMID: 27974827

Note

For research use only.

Images

Reférences

Fiche technique

Anti-PPP3CA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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