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Anti-pptT Polyclonal Antibody (JN126014)

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Vista general
Número de catálogoJN126014
Descripción
Anti-pptT Polyclonal Antibody (JN126014) is a rabbit polyclonal antibody detecting 4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesMycobacterium tuberculosis
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant pptT (Met1-Leu227).
Objetivo 4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O33336
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

4'-phosphopantetheinyl transferase PptT is a ~24 kDa protein. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. Involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors. Acts on Pks13, Mas, PpsA, PpsB, PpsC and PpsD. Also acts on AcpM, the meromycolate extension acyl carrier protein. In addition, is involved in the activation of the acyl carrier protein MbtL and the nonribosomal peptides synthases MbtB and MbtE, which are involved in the biosynthesis of the siderophore mycobactin.

1. Chalut, C. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8511-6. PMID: 16709676
2. Zimhony, O. et al. (2015) Biochemistry 54, 2360-71. PMID: 25785780
3. Jung, J. et al. (2016) FEBS open bio 6, 1220-1226. PMID: 28203522
4. Quadri, LE. et al. (1998) Chemistry & biology 5, 631-45. PMID: 9831524
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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