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Anti-PRV-A NSP2 Polyclonal Antibody (VK105024)

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Vista general
Número de catálogoVK105024
Descripción
Anti-PRV-A NSP2 Polyclonal Antibody (VK105024) is a rabbit polyclonal antibody detecting NSP2 in ELISA, IHC, WB. Suitable for Rotavirus A.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesRotavirus A (RV-A)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant PRV-A NSP2 (Met1-Ile317).
Objetivo Non-structural protein 2, NSP2, 3.6.4.-, NCVP3, Non-structural RNA-binding protein 35, NS35
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso D5LJN4, B3SRX0, Q03242, A2T3M2, B3SRS2, B3SRQ6, Q86505, Q03243, B3SRX8, B3SRR4, A4ZCW3, P09366, B2BRG3, P03537, P03538, Q03241, Q03245, A2T3P0, A3DSK8, Q9QNA8, Q3ZK62, B3SRV4, Q03240
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Non-structural protein 2 (NSP2) is a ~36 kDa protein. Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms), which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.

1. Dhillon, P. et al. (2018) Journal of virology 92. PMID: 30258011
Nota For research use only
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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