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Anti-PTGIS Polyclonal Antibody (MS772014)

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Vista general
Número de catálogoMS772014
Descripción
Anti-PTGIS Polyclonal Antibody (MS772014) is a rabbit polyclonal antibody detecting Prostacyclin synthase; 5.3.99.4; Hydroperoxy icosatetraenoate dehydratase; 4.2.1.152; Prostaglandin I2 synthase; Ptgis; Cyp8 in ELISA, IHC, WB. Suitable for Mouse, Human, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesMouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant mouse PTGIS (Gly42-Leu501).
Objetivo Prostacyclin synthase; 5.3.99.4; Hydroperoxy icosatetraenoate dehydratase; 4.2.1.152; Prostaglandin I2 synthase; Ptgis; Cyp8
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q16647, O35074, Q62969
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Prostacyclin synthase (PTGIS) is a ~57 kDa protein. Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).

1. Nakayama, T. et al. (2002) Biochemical and biophysical research communications 297, 1135-9. PMID: 12372404
2. Wada, M. et al. (2004) Journal of biochemistry 135, 455-63. PMID: 15115769
3. Li, YC. et al. (2008) The Journal of biological chemistry 283, 2917-26. PMID: 18032380
4. Cho, SA. et al. (2015) Archives of biochemistry and biophysics 569, 10-8. PMID: 25623425
5. Yeh, HC. et al. (2007) Archives of biochemistry and biophysics 461, 159-68. PMID: 17459323
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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