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Anti-PV-1 Protease 2A Polyclonal Antibody (VK557024)

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概要
カタログ番号VK557024
説明
Anti-PV1 Protease 2A Polyclonal Antibody (VK557024) is a rabbit polyclonal antibody detecting Protease 2A in ELISA, IHC, WB. Suitable for Poliovirus type 1, Poliovirus type 2, and Poliovirus type 3.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Poliovirus type 1 (PV1), Poliovirus type 2 (PV2), Poliovirus type 3 (PV3)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant PV1 Protease 2A (Gly882-Gln1030).
ターゲット Genome polyprotein, P1, Capsid protein VP0, VP4-VP2, Capsid protein VP4, P1A, Virion protein 4, Capsid protein VP2, P1B, Virion protein 2, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, P2, Protease 2A, P2A, 3.4.22.29, Picornain 2A, Protein 2A, Protein 2B, P2B, Protein 2C, P2C, 3.6.1.15, P3, Protein 3AB, Protein 3A, P3A, Viral protein genome-linked, VPg, Protein 3B, P3B, Protein 3CD, 3.4.22.28, Protease 3C, 3.4.22.28, Picornain 3C, P3C, RNA-directed RNA polymerase, RdRp, 2.7.7.48, 3D polymerase, 3Dpol, Protein 3D, 3D
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Purified by antigen affinity column.
アクセッション番号 P06210, P23069, P03300, P06209, P03301, P03302
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Genome polyprotein (Protease 2A) is a ~245 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

注意事項 For research use only
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参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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