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Anti-PYGB Polyclonal Antibody (HY073014)

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Vista general
Número de catálogoHY073014
Descripción
Anti-PYGB Polyclonal Antibody (HY073014) is a rabbit polyclonal antibody detecting PYGB in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Ovis aries.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PYGB (Pro477-Glu731).
Objetivo Glycogen phosphorylase, brain form, PYGB
Purificación Purified by antigen affinity column.
Número de acceso P11216
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Glycogen phosphorylase, brain form (PYGB) is a ~96 kDa protein. Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

1. Mathieu, C. et al. (2016) The Journal of biological chemistry 291, 18072-83. PMID: 27402852
2. Newgard, CB. et al. (1988) The Journal of biological chemistry 263, 3850-7. PMID: 3346228
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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