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Anti-RAD21 Polyclonal Antibody (HA549014)

Anti-RAD21 Polyclonal Antibody
Anti-RAD21 Polyclonal Antibody
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Aperçu
Numéro de catalogueHA549014
Description
Anti-RAD21 Polyclonal Antibody (HA549014) is a rabbit polyclonal antibody detecting RAD21 in WB, IHC, ELISA. Suitable for Human, Mus musculus, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsWB, IHC, ELISA
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant RAD21 (Glu147-Thr349).
Cible RAD21
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès O60216
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsDouble-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, 64-kDa C-terminal product, 64-kDa carboxy-terminal product, 65-kDa carboxy-terminal product, RAD21
Contexte

Double-strand-break repair protein rad21 homolog is a ~71 kDa protein. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter.

1. Hauf, S. et al. (2001) Science (New York, N.Y.) 293, 1320-3. PMID: 11509732
2. Gregson, HC. et al. (2001) The Journal of biological chemistry 276, 47575-82. PMID: 11590136
3. Bonora, E. et al. (2015) Gastroenterology 148, 771-782.e11. PMID: 25575569
Note For research use only.
Images
  • Anti-RAD21 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RAD21 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: K562 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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