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Anti-RALA Polyclonal Antibody (HY266014)

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Aperçu
Numéro de catalogueHY266014
Description
Anti-RALA Polyclonal Antibody (HY266014) is a rabbit polyclonal antibody detecting RALA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Saguinus oedipus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human RALA (Met1-Leu206).
Cible Ras-related protein Ral-A, RAL, RALA
Purification Purified by antigen affinity column.
Numéro d'accès P11233
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Ras-related protein Ral-A (RALA) is a ~23 kDa protein. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor.

1. Cascone, I. et al. (2008) The EMBO journal 27, 2375-87. PMID: 18756269
2. Aziziyeh, AI. et al. (2009) Cellular signalling 21, 1207-17. PMID: 19306925
3. Balasubramanian, N. et al. (2010) Current biology : CB 20, 75-9. PMID: 20005108
4. Kashatus, DF. et al. (2011) Nature cell biology 13, 1108-15. PMID: 21822277
5. Hiatt, SM. et al. (2018) PLoS genetics 14, e1007671. PMID: 30500825
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RALA Polyclonal Antibody

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