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Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody (RB650014)

Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody
Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody
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Aperçu
Numéro de catalogueRB650014
Description
Anti-Rat CD13/ANPEP/APN Polyclonal Antibody (RB650014) is a rabbit polyclonal antibody detecting ANPEP in ELISA, IHC, WB. Suitable for Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesRat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Rat CD13/ANPEP/APN (Thr75-Ser280).
Applications testées WB
Cible ANPEP, AP-M, AP-N, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, CD13, EC:3.4.11.2, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150, hAPN
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P15684
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Aminopeptidase N (CD13/ANPEP) is a ~108 kDa protein. Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide.

1. Terashima, H. et al. (1995) Journal of gastroenterology 30, 696-704. PMID: 8963385
Note For research use only.
Images
  • Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ANPEP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: PC-3 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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