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Anti-Rat DIO1 Polyclonal Antibody (RW476014)

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Aperçu
Numéro de catalogueRW476014
Description
Anti-Rat DIO1 Polyclonal Antibody (RW476014) is a rabbit polyclonal antibody detecting DIO1. Suitable for Rat and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesMouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Rat DIO1 (Arg85-Phe257).
Cible 5DI, DIO1, DIOI, EC:1.21.99.3, EC:1.21.99.4, ITDI1, TXDI1, Type 1 DI, Type I iodothyronine deiodinase, Type-I 5'-deiodinase
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P24389
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Type I iodothyronine deiodinase (DIO1) is a ~29 kDa protein. Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the deiodination of T4 to 3,3',5'-triiodothyronine (rT3), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine(3-T1) and 3,3'-diiodothyronine (3,3'-T2) to 3-T1 via inner-ring deiodination (IRD). Catalyzes the deiodination of rT3 to 3,3'-T2 via ORD. Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine, 3',5'-diiodothyronamine and 3,3'-diiodothyronamine as well as tyrosyl ring deiodinations of 3,5,3'-triiodothyronamine and 3,5-diiodothyronamine.

1. Berry, MJ. (1992) The Journal of biological chemistry 267, 18055-9. PMID: 1517238
2. Leonard, JL. et al. (1991) The Journal of biological chemistry 266, 11262-9. PMID: 2040632
3. Toyoda, N. et al. (1995) The Journal of biological chemistry 270, 12310-8. PMID: 7744884
4. Berry, MJ. et al. (1991) Nature 349, 438-40. PMID: 1825132
5. Moreno, M. et al. (1994) FEBS letters 344, 143-6. PMID: 8187873
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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