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Anti-RBM33 Polyclonal Antibody (HB545014)

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Aperçu
Numéro de catalogueHB545014
Description
Anti-RBM33 Polyclonal Antibody (HB545014) is a rabbit polyclonal antibody detecting RNA-binding protein 33; Proline-rich protein 8; RNA-binding motif protein 33; RBM33; PRR8 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human RBM33 (Gln1096-Glu1170).
Cible RNA-binding protein 33; Proline-rich protein 8; RNA-binding motif protein 33; RBM33; PRR8
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q96EV2, Q9CXK9
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

RNA-binding protein 33 (RBM33) is a ~129 kDa protein. RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as mRNA export, mRNA stability and/or translation. Binds a subset of intronless RNAs containing GC-rich elements, such as NORAD, and promotes their nuclear export by recruiting target RNAs to components of the NXF1-NXT1 RNA export machinery. Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, promoting their demethylation by ALKBH5. Acts as an molecular adapter, which (1) promotes ALKBH5 recruitment to m6A-containing transcripts and (2) activates ALKBH5 demethylase activity by recruiting SENP1, leading to ALKBH5 deSUMOylation and subsequent activation.

1. Thomas, A. et al. (2022) Genes & development 36, 550-565. PMID: 35589130
2. Yu, F. et al. (2023) Molecular cell 83, 2003-2019.e6. PMID: 37257451
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-RBM33 Polyclonal Antibody

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