Anti-RHO Polyclonal Antibody (HY021014)

Name: Anti-RHO Polyclonal Antibody
Brand: abinScience
SKU: HY021014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HY021014

Description

Anti-RHO Polyclonal Antibody (HY021014) is a rabbit polyclonal antibody detecting Rhodopsin; Opsin-2; RHO; OPN2 in ELISA, IHC, WB. Suitable for Human, Dog, Pig, Cat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human RHO (Asn2-Gln36&Thr97-Cys110&Gly174-Ser202).

Cible

Rhodopsin; Opsin-2; RHO; OPN2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P08100, P32308, O18766, Q95KU1, P15409, P02700, P51489, P02699, P29403, P28683, P49912

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071

2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847

3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452

4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617

5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343

6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165

7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425

Note

For research use only

Images

Reférences

Fiche technique

Anti-RHO Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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