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Anti-Rice CENH3 Polyclonal Antibody (PR109024)

Anti-Rice CENH3 Polyclonal Antibody
Anti-Rice CENH3 Polyclonal Antibody
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Vista general
Número de catálogoPR109024
Descripción
Anti-Rice CENH3 Polyclonal Antibody (PR109024) is a rabbit polyclonal antibody detecting Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesOryza sativa (Rice)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant rice CENH3 (Gly71-Trp164).
Objetivo Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q6T367
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Histone H3-like centromeric protein CENH3 is a ~18 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (Probable).

1. Nagaki, K. et al. (2004) Nature genetics 36, 138-45. PMID: 14716315
2. Yan, H. et al. (2006) The Plant cell 18, 2123-33. PMID: 16877494
Nota For research use only
Imágenes
  • Anti-Rice CENH3 Polyclonal Antibody

    Western blot

    Lane 1: HCT116 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Raji cell lysate
    Lane 6: Hela cell lysate
    Lane 7: A375 cell lysate
    Lane 8: A431 cell lysate
    Lane 9: K562 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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