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Anti-Rice CENH3 Polyclonal Antibody (PR109024)

Anti-Rice CENH3 Polyclonal Antibody
Anti-Rice CENH3 Polyclonal Antibody
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概要
カタログ番号PR109024
説明
Anti-Rice CENH3 Polyclonal Antibody (PR109024) is a rabbit polyclonal antibody detecting Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Oryza sativa (Rice)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant rice CENH3 (Gly71-Trp164).
ターゲット Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 Q6T367
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Histone H3-like centromeric protein CENH3 is a ~18 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (Probable).

1. Nagaki, K. et al. (2004) Nature genetics 36, 138-45. PMID: 14716315
2. Yan, H. et al. (2006) The Plant cell 18, 2123-33. PMID: 16877494
注意事項 For research use only
イメージ
  • Anti-Rice CENH3 Polyclonal Antibody

    Western blot

    Lane 1: HCT116 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Raji cell lysate
    Lane 6: Hela cell lysate
    Lane 7: A375 cell lysate
    Lane 8: A431 cell lysate
    Lane 9: K562 cell lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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