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Anti-RNMT Polyclonal Antibody (HB576014)

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Vista general
Número de catálogoHB576014
Descripción
Anti-RNMT Polyclonal Antibody (HB576014) is a rabbit polyclonal antibody detecting mRNA cap guanine-N7 methyltransferase; 2.1.1.56; RG7MT1; mRNA (guanine-N(7))-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p; RNMT; KIAA0398 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human RNMT (Gly144-Gln476).
Objetivo mRNA cap guanine-N7 methyltransferase; 2.1.1.56; RG7MT1; mRNA (guanine-N(7))-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p; RNMT; KIAA0398
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O43148, Q5U2U7, Q9D0L8
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

mRNA cap guanine-N(7) methyltransferase (RNMT) is a ~54 kDa protein. Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.

1. Saha, N. et al. (1999) The Journal of biological chemistry 274, 16553-62. PMID: 10347220
2. Wen, Y. et al. (2000) Genes & development 14, 2944-9. PMID: 11114884
3. Gonatopoulos-Pournatzis, T. et al. (2011) Molecular cell 44, 585-96. PMID: 22099306
4. Varshney, D. et al. (2016) Nucleic acids research 44, 10423-10436. PMID: 27422871
5. Pillutla, RC. et al. (1998) The Journal of biological chemistry 273, 21443-6. PMID: 9705270
6. Tsukamoto, T. et al. (1998) Biochemical and biophysical research communications 251, 27-34. PMID: 9790902
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-RNMT Polyclonal Antibody

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