Anti-RPA1 Polyclonal Antibody (HA686014)

Name: Anti-RPA1 Polyclonal Antibody
Brand: abinScience
SKU: HA686014
Price: 96 USD
Availability: InStock

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Spécification :

20ug
50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HA686014

Description

Anti-RPA1 Polyclonal Antibody (HA686014) is a rabbit polyclonal antibody detecting N-terminally processed; RPA1; REPA1; RPA70 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human RPA1 (Ile187-Met616).

Cible

Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P27694, Q8VEE4

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Replication protein A 70 kDa DNA-binding subunit (RPA1) is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717

3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720

4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682

5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808

6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923

7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716

8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279

9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650

10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-RPA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate

Reférences

Fiche technique

Anti-RPA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-RPA1 Polyclonal Antibody (HA686014)

Aperçu

Images

Reférences

Fiche technique

SDS