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| カタログ番号 | HA686014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-RPA1 Polyclonal Antibody (HA686014) is a rabbit polyclonal antibody detecting N-terminally processed; RPA1; REPA1; RPA70 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant human RPA1 (Ile187-Met616). | ||||||||
| ターゲット | Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P27694, Q8VEE4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Replication protein A 70 kDa DNA-binding subunit (RPA1) is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. 1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542 2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717 3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720 4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682 5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808 6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923 7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716 8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279 9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650 10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-RPA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate
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