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Anti-RV-A VP6 Polyclonal Antibody (VK021014)

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概要
カタログ番号VK021014
説明
Anti-RV-A VP6 Polyclonal Antibody (VK021014) is a rabbit polyclonal antibody detecting Intermediate capsid protein VP6 in ELISA, IHC, WB. Suitable for Rotavirus A.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Rotavirus A (RV-A)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant RV-A VP6 (Met1-Lys397).
ターゲット Intermediate capsid protein VP6
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 Q86219, A7J3A1, A7J3A5, P03531, B1NKU0, B1NKS8, Q86347, B1NKU4, P16488, A4ZCW7, P03530, Q86345, Q91N56, B1NKQ8, P16593, P16592, B1NKR2, P04509, Q06386, B1NKR6, B1NKT2, Q89601, Q9QNB0, P89043, P16487, P18610, A2T3T0, Q3ZK59, P87723, A7J3A8, Q8JTI5, P08035, Q6PMI4, Q6LE89, B2BN53, Q91N61
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Intermediate capsid protein VP6 is a ~44 kDa protein. Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.

注意事項 For research use only
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参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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